Poster RNAseq Rocket
The implementation of rapid and non-destructive methods is necessary for the evaluation of quality in ready-to-eat leafy vegetables during harvest, postharvest and storage stages. Since the quality after harvest cannot be improved but only preserved, quality markers must be isolated in order to identify the minimum variation of quality already after the first day of storage.
The aim of our research is to isolate molecular markers for the quality assessment of rocket (Diplotaxis tenuifolia L.) by applying transcriptome sequencing (RNA-seq).
Rocket plants were grown using hydroponic systems in controlled growth chambers for 3 weeks prior to stress treatment. In pre-harvest was imposed salinity (200?????mM NaCl), heat radical stress (40 C), nitrate deficiency (absence of NH4+/NO3-), while in post-harvest was imposed chilling (4 C), mechanical wounding of leaves, dark and water stress. Sampling was performed after 24 hours in order to identify early-responsive and late-responsive genes activated by each stress. Total RNA was extracted from stressed and control plants and each sample was sequenced using the Illumina HiSeq 2000 platform. The RNA-seq analysis allowed to identify interesting candidate genes that are specifically expressed in each stress as well as genes that are commonly expressed among all the stresses under study. These genes encode for proteins associated with senescence, degenerative processes and quality loss. The common genes expressed among all stress treatments will be considered as quality molecular markers and their expression levels will be analyzed under several conditions during the storage chain. The expression of putative markers are correlated with physiological and biochemical parameters.